RNase HII, Glycerol-free(2 U/μL) _ 14541ESRNase HII is a ribonuclease internal enzyme obtained from the Pyrococcus abyssi and recombinantly expressed in E. Coli. It recognizes the DNA rN DNA DNA double strand, cutting at the site where ribonucleotides are incorporated into DNA. However, its activity on single stranded RNA is very low, and it shows no cutting activity on dsDNA or ssDNA. RNase HII cuts at the site of a single ribonucleotide residue from the 5' end, producing a 5' phosphate

After completely thawed

Sorting usually requires two rounds of magnetic bead separation

1) Thaw the RCL DNA control and DNA dilution buffer on ice

N1-Me-Pseudo UTP sodium solution is one of the most commonly used modified nucleoside triphosphates

RNase R serves as a fundamental component in circular RNA synthesis (circRNA)

Wide input range compatibility

and the concentration of other fragments is 50 ng/5μL

Molecular Weight Approximately 12 kDa under reducing conditions

1 unit: The amount of enzyme required to digest 1 μg of substrate DNA within 1 h at 37℃ in a 50 μL system

including Hieff™ Taq DNA Polymerase (Cat#10101)

this product is only for reference when judging the molecular weight of the target protein

[26] Xie WJ